|
To document that effects of hyperbaric oxygen(HBO) and α-tocopherol on full-thickness skin grafts in rat, we performed full-thickness skin grafts bilaterally on each rats. The HBO-treated rats were received HBO twice daily for 90 minutes at 2 ATA. Surgical control rats were not treated with HBO. α-tocopherol treated rats were received the agent via oral gastric tube daily for 3 days preoperative and a fourth dose 1 to 2 hours postoperative. HBO plus α-tocopherol treated rats were received HBO and α-tocopherol as mentioned above. Biopsy specimens were taken from each rat at the time of grafting and on days 2, 4, 7, 10, 14, 21, and 28, then were processed for tissue-concentration of total glutathione(GSHt), oxidized/reduced glutathione level, and thiobarbituric acid-reactive substance(TBARS) levels. The percentage of viable graft on day 10 ranged from 67 to 93%, and was not significantly different among the each other groups. The percentage of viable graft were, however, higher in HBO plus α-tocopherol treated rats(78.6%) than in HBO alone treated rats(59.1%), α-tocopherol alone treated rats(66.7%) and surgical control rats(58.2%). TBARS concentration had a significant increase from preoperative concentration at day 2, and peak concentration at day 4(p<0.01). Concentration then decreased to preoperative concentration at day 28. GSHt concentration of free skin graft had a similar patteren of change in four groups and decreased significantly from preoperative concentration at day 2, returning to preoperative concentration by day 7(surgical control, HBO-treated, and α-tocopherol-treated, alone) and 28(HBO plusα-tocopherol-treated). Percentage of the concentration of reduced glutathione decreased in surgical control, HBO-treated and, α-tocopherol-treated(p<0.05), and HBO plus α-tocopherol-treared(p<0.01) on day 7 after surgery, whereas the concentration of oxidized increased significantly in HBO-treated(p<0.05), α-tocopherol-treated(p<0.05), and HBO plus α-tocopherol-treated(p<0.01).
|